Channel: Science & Technology
Uploaded: December 31, 1969 at 11:59 pm
Author: dlindeman
Length: 00:02
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Views: 41
A HIGH-RESOLUTION IN VITRO BIOASSAY TO IDENTIFY NEURONS CONTAININGRED PIGMENT CONCENTRATING HORMONERAMÓN ALVARADO-ÁLVAREZ, ELIZABETH BECERRA AND UBALDO GARCÍA*Fig. 6. Correlation between evoked neuronal firing and aggregation of the pigmentary matrix in erythrophores. (A) Co-cultured cells after 48 h.Note that the pole of the erythrophore adjacent to the neurons is retracted. Trains of action potentials evoked by intracellular current injectioninto the neuron for 5 min (illustrated in H) induced progressive aggregation of the pigmentary matrix. (B) The retraction occurred mainly infilipodia next to the neuron (this photograph was taken at the end of electrical stimulation). (C) The aggregation continued throughout the cell,even in the absence of neuronal firing (photograph taken at 10 min). (D) The erythrophore recovered its shape after superfusion with freshmedium for 12 h. (E,F) Stimulation of the second neuron (illustrated in I) reproduced the effect on the target cell, which again recovered itsshape after superfusion with fresh medium (G). Scale bar, 50 mm. Em, membrane potential.